Impact of Resveratrol in Attenuating Cisplatin Induced Testicular Toxicity in Male adult Rats

Shahnaz Memon; Sajjad Ali Almani; Muhammad Saqib Baloch; Samreen Ali; Tanveer Ahmed Talpur; Harender Kumar

doi:10.35787/jimdc.v11i1.716

Shahnaz Memon Assistant Professor, Department of Pharmacology, Isra University, Hyderabad
Sajjad Ali Almani Assistant professor, Department of Anatomy, Dow university of Health & Science
Muhammad Saqib Baloch Assistant Professor, Department of Anatomy, Muhammad medical college, Mirpurkhas
Samreen Ali Assistant Professor, Department of Pharmacology, Suleman Roshan Medical College
Tanveer Ahmed Talpur Final year Medical Student, Isra University, Hyderabad
Harender Kumar Final year Medical Student, Isra University, Hyderabad

DOI: https://doi.org/10.35787/jimdc.v11i1.716

Keywords: Cisplatin, Oxidative Stress, Resveratrol, Toxicity

Abstract

Background: Resveratrol is a poly-hydroxy phenol plant toxin that reduces oxidative stress and prevents tissue damage by increasing endogenous antioxidant levels. The study aimed to investigate the protective effects of Resveratrol by histochemical, ultrastructural and biochemical methods in testicular toxicity induced by the Cisplatin in Wistar Albino rats.

Methodology: The quasi-experimental study was carried out at the department of Pharmacology, Anatomy and Postgraduate Laboratory of ISRA University Hyderabad from October 2020 to March 2021. Twenty-four male, healthy Wistar Albino rats of age from 8-10 weeks and having body weight 250-300 grams, were included in the study. Rats were divided into three groups; each group have same number (n=08) of rats. Group-A (Control), Group-B (Experimental group or Cisplatin group), Group-C (Experimental group or Cisplatin + Resveratrol combination group). Pre- and post-experimental body weight of all animals was measured, blood samples were collected for the biochemical analysis for the oxidative markers, semen parameters, and histo-morphology. Data was analyzed using SPSS version 24.0.

Results: Statistically significant decline in the bodyweight and testicular weight in group B and C respectively (p<0.05). While Group-B had lower sperm count, motility, and viability when compared to Group-C (p<0.05). Group-B also had significantly lower levels of oxidative markers than Group-C (p<0.05). Group- B's testicular histology significantly differed from Group-C's (p<0.05). Seminiferous tubules in Group-B were irregular, regressive, and atrophic.

Conclusion: Resveratrol is a powerful antioxidant shows potential in reducing cisplatin-induced oxidative stress and, eventually, testicular toxicity in mice models.